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. Author manuscript; available in PMC: 2011 Oct 1.
Published in final edited form as: J Neurovirol. 2010 Oct;16(5):384–398. doi: 10.3109/13550284.2010.515651

Figure 5.

Figure 5

Native and alkaline gel analyses of HSV-1 DNA derived from quiescently infected NGF-PC12 cultures. (A) Outline of experimental procedure. (B) Southern blot analysis of HSV-1 DNA derived from infected NGF-PC12 cultures that was prepared and quantified as described in Figure 3. HSV-1 DNA (25 ng) from each sample was digested with restriction endonuclease BamHI. One-third of the digest was resolved on a TAE gel and the other two-thirds was resolved on an alkaline gel, transferred to nylon membranes, hybridized with 32P-labeled HSV BamHI B/E probe, scanned, and quantified using the Bio-Rad phosphoimager. (C) The ratio of the amount of HSV-1 BamHI B/E DNA detected in the native and alkaline gels. The quantity of HSV-1 DNA in indicated samples shown in panel B was quantified using the virion DNA standard. The ratio of quantified HSV DNA between the TAE gel and alkaline gel is calculated. At bottom, the ratio values were normalized so that the D0 sample ratio is set to 1.