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. 2009 Jun;19(2):117–128. doi: 10.1089/oli.2008.0177

FIG. 3.

FIG. 3.

Aptamers WT-15 and SM-20 disrupt the PAI-1/thrombin/heparin complex without affecting the PAI-1/tPA complex. (A) SM-20 and WT-15 disrupt the thrombin/PAI-1 complex in a concentration-dependent manner. PAI-1 (500 nM) was incubated with WT-15 (lanes 5 and 8), SM-20 (lanes 6 and 9), or Sel2 (lanes 7 and 10) (500–1000 nM) for 10 minutes before addition of thrombin (200 nM) and heparin (10 μg/mL). Lane 1: MW marker; lane 2: PAI-1 alone; lane 3: thrombin alone; lane 4: PAI-1/thrombin/heparin; lanes 5, 6, 7: aptamers at 500 nM; lanes 8, 9, 10: aptamers at 1000 nM. (B) The aptamers are unable to disrupt the PAI-1/tPA complex. PAI-1 (500 nM) was incubated with WT-15, SM-20, or Sel 2 (500 nM) prior to adding tPA (400 nM). Lane 1: MW marker; lane 2: PAI-1 alone; lane 3: tPA alone; lane 4: PAI-1/tPA; lane 5: PAI-1/tPA/WT-15; lane 6: PAI-1/tPA/SM-20; lane 7: PAI-1/tPA/Sel2.