FIG. 1.

(A) Schematic of the target region encompassing HPRT1 Exon 3 and the PCR strategy for generating the 579-bp targeting SDF. The strategy for the assessment of genomic DNA targeting as well as the RFLP analysis of the 1025-bp diagnostic product is also depicted. The sequence represents Exon 3 and the LT1-1B1 cell line 152G>C (R51P) mutation. The mutation is indicated as (“g” = 152 G > C). (B) Schematic representation of the RT-PCR and RFLP analysis of HPRT1 mRNA. The XhoI digestion for the 256-bp amplicon will result in a 190- and 66-bp fragment.