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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: Biomed Microdevices. 2010 Jun;12(3):533–541. doi: 10.1007/s10544-010-9410-9

Fig. 6.

Fig. 6

(a) Performance of the droplet-based regular TaqMan® PCR (▲) and the nested TaqMan® PCR in a well-mixed droplet (■) and diffusively mixed droplet (●) through 45 cycles. The dashed line represents the fluorescence threshold (1.13). (b) Fluorescent image after 31 cycles of regular TaqMan® PCR in a droplet. (c)–(d) Fluorescent images of nested TaqMan® PCR in a well-mixed and diffusively mixed droplet, respectively. For the images, the two steps in nested TaqMan® PCR were carried out for 15 and 16 cycles, respectively. The original concentration of template λDNA is 3.5 × 10−5 ng/µL (~6.69 × 102 copies/µL) for both regular and nested TaqMan® PCR. The error bar represents the standard deviation. The scale bar represents 400 µm