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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Alcohol Clin Exp Res. 2010 Jun 25;34(9):1603–1612. doi: 10.1111/j.1530-0277.2010.01245.x

Fig. 7.

Fig. 7

Adult corticotropin-releasing factor (CRF) immunoreactivity within the central nucleus of the amygdala (CeA) and paraventricular nucleus of the hypothalamus (PVN) following repeated ethanol withdrawal and stress/withdrawal paradigms (Panels A & B). Adult rats were given either 19 days of control diet (CD), three 5-day cycles of 3.5% ethanol diet (ED) interspersed with two 2-day withdrawal periods (3.5% CY3), or 14 days of CD followed by 5 days of 3.5% ED with stress (3.5% CY1-Str). Stress was one hour of restraint stress given at weekly intervals. Brain tissue was collected immediately following social interaction 5 hours into ethanol withdrawal. Total density measurements were taken using ImageJ program for CeA and PVN. Data represent means ± SEM for 8 rats/group. “*” is significantly different from control (p < 0.05).