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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: J Alzheimers Dis. 2010 Jan;19(4):1205–1219. doi: 10.3233/JAD-2010-1314

Figure 2.

Figure 2

Activation of GLPR-1 protects human neuroblastoma cells against oxidative injury. A) One step RT-PCR shows human GLP-1R mRNA expression in human (h) SH-SY5Y neuroblastoma cells. M: DNA marker; lanes 1 and 2: RT-PCR of hGLP-1R from 0.5 and 1 μg total RNA extracted from SH-SY5Y cells, hGLP-1R 1f/1r primers were used, which amplify a 480 bp product; lane 3: negative control. B) GLP-1 (10 nM), stimulated the release of cAMP from cultures SH-SY5Y cells (N=3, **p<0.01 (Student’s t-test) 15 min vs. zero time). C) Pretreatment with Ex-4 protected SH-SY5Y cells from H2O2 (50≥M)-induced loss of cell viability, assessed by MTS. Compared to controls, 2 h exposure to H2O2 induced a 15% loss of cell viability, which was completely ameliorated by Ex-4 (100 nM). This effect was abolished by the GLP-1R antagonist, exendin-9-39 (Ex-9-39, 10 ≥M) (N≥5 for each treatment, p< 0.05 (Bonferroni t-test).