Fig. 2.

Obatoclax augments TRAIL-mediated apoptotic signaling. A, PANC-1 and BxPC-3 cells were treated with obatoclax and/or TRAIL for 24 h and apoptosis was determined by phosphatidylserine externalization as detected by Annexin V-APC binding using flow cytometry. Annexin V-positive cells were quantitated as described in Materials and Methods. Results of triplicate determinations are shown. B, combination of obatoclax and TRAIL enhances the cleavage of caspase-8, -9, and -3 and reduces full-length Bid in PANC-1 and BxPC-3 cell lines. Cells were treated with drugs for 6 or 12 h and whole-cell lysates were prepared and then subjected to immunoblot analysis for determination of protein expression. β-Tubulin served as a loading control.