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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Clin Cancer Res. 2009 Jan 1;15(1):150–159. doi: 10.1158/1078-0432.CCR-08-1575

Fig. 5.

Fig. 5

Obatoclax displaces proapoptotic Bak and Bim from their sequestration by prosurvival Mcl-1, Bcl-xL, or Bcl-2 proteins. PANC-1 (A) and BxPC-3 (B) cells were treated with obatoclax or diluent (DMSO) for 24 h and Bak proteins were immunoprecipitated. Precipitated proteins were then subjected to immunoblot analysis for the designated proteins. Whole-cell lysates and IgG heavy chain indicate similar protein loading. PANC-1 (C) and BxPC-3 (D) cells were treated with obatoclax or diluent (DMSO) for 24 h and Bim splice variants were immunoprecipitated. Precipitated proteins were then subjected to immunoblot analysis for the designated proteins. Whole-cell lysates and IgG heavy chain were also shown for similar loading for each condition. β-Tubulin served as a loading control.