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. 2010 Oct 1;5(10):e13119. doi: 10.1371/journal.pone.0013119

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Seinen et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A: Schematic presentation of the event in which identical phenotypes are induced because of shared on-target effects and at the same time different phenotypes are induced because of off-target effects. Phenotype 2 is due to down-regulation of the on-target gene and is induced by dsRNA1 and dsRNA2. Phenotype 1 and 3 are due to down regulation of the off-target gene X and Y respectively and are specific for the individual distinct dsRNAs. In this fortunate event, the individual off-target effects are not identical and are classified as off-target-effects; bona fide conclusions will be drawn from the outcome of this experiment. B: Schematic presentation of the event in which identical phenotypes are induced because of shared on-target effects but at the same time an additional identical phenotype is induced by the use of the two independent dsRNAs caused by off-target effects. Phenotype 2 is due to down-regulating the on-target gene and is shared by dsRNA1 and dsRNA3. Phenotype 1 is due to down regulation of a shared off-target gene of the distinct dsRNAs. In this unfortunate event, the off-target effects are identical and will be classified as on-target effects; false conclusions will be drawn from the outcome of this experiment.