Figure 6. Non-overlapping cDNA sequences of randomly chosen genes have a significant prevalence of sharing off-targets in exon derived sequences of other genes.

All 99 cDNA sequences were re-analyzed, now ignoring any predicted off-targets that occur within intron sequences. Although this lowers the predicted off-targets, it still shows a significant occurrence of overlapping off-targets. Our analysis shows that after applying this filter, 74% of the genes contain non-overlapping cDNA sequences that share sequence similarity with the same off-target (see also Supporting Information S1).