Figure 4. Cytokine production of human CD8⁺ T cells reconstituted in hNOK mice.

Splenocytes from hNOK mice were cultured with PMA and ionomycin for 6 hours and then the production of IFN-γ, TNF-α, and IL-2 cytokines by human CD8⁺ T cells was measured by flow cytometry. (A) A representative result for cytokine production by human CD8⁺ T cells from a hNOK mouse (lower data) and a human adult individual (upper data). (B) Correlation between frequency of IFN-γ-, TNF-α-, and IL-2⁺IFN-γ⁻TNF-α⁻ (IL-2^single+)-producing CD8⁺ T cells and that of effector memory (CD27⁺CD28⁺CCR7⁻CD45RA⁻ plus CD27^lowCD28⁻CD45RA^+/−CCR7⁻) CD8⁺ T cell subset (n = 12).