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. 2010 Oct 1;5(10):e13109. doi: 10.1371/journal.pone.0013109

Figure 5. Alloreactivity of human CD8+ T cells reconstituted in hNOK mice.

Figure 5

The hNOK mice were immunized for 31 days with irradiated human PBMC (allo-hPBMC) from a healthy donor with HLA-A*2402/A*2402, HLA-B*5201/B*5901, and HLA-DRB1*1502/DRB1*0405 (immunized mice) or PBS (un-immunized mice). The splenocytes of each hNOK mice were harvested, and the cells were stained by using anti-human CD45, anti-human CD3, and anti-human CD8 mAbs. (A) Human adult PBMC from a healthy donor with HLA-A*2601/A*2403, HLA-B*3501/B*5101, and HLA-DRB1*0405/DRB1*0405 were cultured with the allo-hPBMC or the self-hPBMC for 7 days in vitro, and a representative result showing the IFN-γ production by the human CD8+ T cells and the human CD8− T cells is shown as a control (upper data). The splenocytes from the immunized hNOK mice were cultured with the allo-hPBMC or self-CB for 7 days in vitro, and a representative result for IFN-γ production by the human CD8+ T cells and the human CD8− T cells is shown (lower data). (B) Splenocytes from the immunized hNOK mice were cultured with irradiated 721.221 cells expressing HLA-A*2402 (.221-A*2402) or irradiated 721.221 expressing HLA-B*5201 (.221-B*5201) for 3 days in vitro, and a representative result for the proliferation of the human CD8+ T cells or the human CD8− T cells is shown. One representative experiment from 3 experiments is shown.