Figure 8. Reactivity of human specific conserved DBL5ε affinity purified antibodies with P. falciparum infected erythrocytes.

(A): IgG recognition of 3D7-DBL5ε peptides library. (B): Mapping of P4 and P13 peptides on DBL5ε model [8]. (C): Senegalese women antibodies were affinity purified on peptides P4 and P13 and tested for reactivity against PAM Beninese parasite isolates. Flow cytometry analysis of human affinity-purified IgG against peptides P4 and P13 against PAM parasite isolates. Each colour shows the reactivity to native parasites with the indicated antibodies. Four isolates were tested with each IgG. Sample without primary antibody (blank), non-exposed women plasma pool, and exposed women plasma pool are used as control respectively.