Figure 4. Protein expression analysis using the µFBI.

Total protein expression and phosphotyrosine-specific expression of seven RTKs were determined by µFBI (A and B) and a normal bead-based assay (C and D). For µFBI, seven mixed capture antibody-coated beads (1,000 beads per analyte) were incubated with tissue lysates (1 µl) for 60 min, mixed biotinylated detection antibodies (1 µl) for 60 min and Strep-PE (1 µl) for 45 min. The MFI value was obtained with a Luminex 100 IS system.