Table 1. Comparison of µFBI and standard bead-based assay.

Procedure	Micro fluidic bead-based assay		Standard bead-based assay		Fold reduction
	Time	Usage per capillary	Time	Usage per well
Pre-wet	10 min	20 µL	5 min	100 µL
Beads		1 µL, 1000beads/analyte		30 µL, 2000beads/analyte
Vacuum	-	-
Sample	60 min	1 µL, 200 ug/ml	60 min	100 µL, 200 ug/ml	100×
3×wash	-	-
Detection antibody	60 min	1 µL, 1×	60 min	30 µL, 1×	30×
3×wash	-	-
Strep-PE	60 min	1 µL, 1×	60 min	30 µL, 1×	30×
3×wash	-	-
Recover beads	3 min	5 µL	-	-
Measurement	80 µL, 80 s, Gate: 7500–15000		80 µL, 80 s, Gate: 7500–15000

-: without this procedure.