Table 1. Relative enzyme activity of the Rv0045c protein toward p-nitrophenyl derivatives at pH 7.0 and 37°C.
| Substrate | Relative activity (%)a |
| p-nitrophenyl acetate (C2) | 62.3 |
| p-nitrophenyl butyrate (C4) | 10.0 |
| p-nitrophenyl caproate (C6) | 100.0 |
| p-nitrophenyl caprylate (C8) | 14.2 |
| p-nitrophenyl laurate (C12) | 12.1 |
| p-nitrophenyl myristate (C14) | 77.9 |
| p-nitrophenyl palmitate (C16) | ND |
ND: Not detectable
a
The specific activity toward p-nitrophenyl caproate (C6) corresponding to 3.5 U/mg protein/min was defined as 100%. And one hydrolase unit is the quantity of enzyme required to increase absorbance by 0.01 units at 405 nm per min.