Figure 8. NF-κB and MAPK signalling in IRAK-4-deficient fibroblasts and PBMC.

Primary fibroblasts (a) and PBMC (b) from the patient and control were exposed to 100 ng/ml each of LPS, IL-1β, or TNF-α, or unstimulated for the times indicated. Western immunoblot analyses were performed on whole cell lysates using antibodies specific for phosphorylated IκB-α, IκB-α, phosphorylated p38, total p38, phosphorylated JNK, and β-actin, and nuclear proteins using antibodies specific for p65. Immunoblots are representative of n≥2 experimental repeats, using n=2 different normal controls.