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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: J Immunol. 2009 Jul 13;183(3):2122–2132. doi: 10.4049/jimmunol.0804187

Figure 10. Necrotic neutrophils and α-defensins protect mice from experimental inflammation.

Figure 10

Sterile peritonitis was induced by injecting thioglychollate, along with either PBS, apoptotic cells (AC), necrotic human neutrophils (NN), LL37, HNP1-3 or mouse necrotic neutrophils (mNN). After 4 hours peritoneal lavages were used to isolate inflammatory GR1+F480+ macrophages (a) and neutrophils (b), which were characterised by FACS and compared to cell numbers in control mice with peritonitis given PBS. Experiment is representative of 2 separate expts with 5 mice per group. (c) In separate experiments resting murine peritoneal macrophages were isolated and stimulated in vitro with CD40/IFN-γ along with α-defensins and supernatants were collected at various timepoints and tested for TNF-α. *** p ≤0.001, * p ≤0.01, error bars =SEM.