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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: J Immunol. 2009 Jul 13;183(3):2122–2132. doi: 10.4049/jimmunol.0804187

Fig 7. Alpha defensins can still inhibit pro-inflammatory cytokine secretion by HMDMs despite infection with whole bacteria.

Fig 7

(a) HMDMs were co-cultured with dead Pseudomonas Aeruginosa PA01 for 24 hours (i) or live bacteria (at an MOI of 10) for 4 hours (ii) after which culture supernatants were collected and cytokine secretion quantitated by ELISA. (b) For the live bacterial experiment HMDMs were lysed after 4 hours and bacteria cultured for a further 18 hours on agar prior to counting the number of live colonies. (c) Mice were injected with 10×6 of live Salmonella enterica serovar Typhimurium and PBS or NN was administered on days 0,1,2,4 and 6. Mice were sacrificed on day 7 and TNF-α was measured in the serum (i). In addition the number of live bacteria retrieved from lysed splenocytes after an overnight culture was calculated following a further 18 hours of culture on agar (ii). *** p ≤0.0004, ** p ≤0.003, * p ≤0.02. error bars =SEM