Skip to main content
. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: J Immunol. 2009 Jul 13;183(3):2122–2132. doi: 10.4049/jimmunol.0804187

Fig 8. Alpha defensins but not cathelicidins inhibit both T cell mediated and LPS mediated activation of macrophages.

Fig 8

HMDM were stimulated with either LPS (a) or CD40L/IFN-γ (CI) [b] and apoptotic neutrophils (N), necrotic neutrophil supernatants (NN) or LL37 at the indicated doses. In separate experiments HNP1-3 (c-d) or purified HNP-1 was titrated into CI or LPS activated HMDMs. The anti-inflammatory potential of synthetically derived HNP-1 that had been linearized was compared to α-defensins using CD40L/IFN-γ (CI) stimulated HMDMs. Culture supernatants were harvested after 18 hours and tested for TNF-α secretion by ELISA. *** p ≤0.0001, ** p ≤0.001, * p ≤0.01.error bars =SEM when compared to HMDMs treated with stimulus alone .