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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Oncogene. 2007 Sep 24;27(12):1805–1811. doi: 10.1038/sj.onc.1210805

FIGURE 3.

FIGURE 3

The RASSF2A promoter is frequently methylated in NSCLC primary tumours but not in ovarian tumours.

(a) Methylation status of the RASSF2A promoter was determined in NSCLC primary tumours using MSP, where M represents methylated-specific PCR and U represents unmethylated-specfic PCR. Methylation was found in 22/50 (44%) primary tumours. Methylation was found in all grades of tumor (I/II = 7/16 (44%); IIIA = 4/9 (44%); IV = 10/23 (44%)).

(b) RASSF2A CpG island methylation was tumour specific. (T represents tumour; N represents corresponding normal lung)

(c) The RASSF2A CpG island promoter region was unmethylated in primary ovarian tumours. OVCA28, an ovarian tumour cell line partially methylated for RASSF2A, acts as a positive control for both M and U sets of primers. RASSF2A CoBRA was also carried out on these samples to confirm the result (data not shown).