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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Oncogene. 2007 Sep 24;27(12):1805–1811. doi: 10.1038/sj.onc.1210805

FIGURE 4.

FIGURE 4

RASSF2A suppresses growth of breast tumour cells.

(a) Colony formation. The number of Geneticin resistant colonies in cells transfected with empty vector was set at 100 %. Values are the mean ± SD of three separate experiments each calculated from triplicate plates.

(b) Immunoblot stained with anti-FLAG (Sigma clone M5) to detect RASSF2A in protein extracts from MCF7 cells stably transfected with pFLAG-RASSF2A (stable clones, Cl.3 and Cl.4).

(c) Anchorage independent growth studies. Growth of MCF7-RASSF2A stable clones (Cl.3 and Cl.4) and MCF7 cells stably transfected with an empty vector were compared using a soft agar assay. The number of Geneticin resistant colonies in cells transfected with empty vector was set at 100 %. Values are the mean ± SD calculated from triplicate plates.

(d) Tumour formation in SCID mice. Over 140 days tumours develop in mice inoculated with MCF7 cells, however they are not observed in mice inoculated with MCF7 cells expressing RASSF2A.