Skip to main content
. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Oncogene. 2007 Sep 24;27(12):1805–1811. doi: 10.1038/sj.onc.1210805

FIGURE 7.

FIGURE 7

Endogenous RASSF2 is localized to the cytoplasm.

Protein from two human lung cancer cell lines was fractionated into nuclear (N) and cytoplasmic (C) fractions by standard CLB buffer protocol, and the blot immunoprobed with anti-RASSF2, then reprobed with anti-alpha tubulin to confirm that the nuclear fraction was free of contaminating cytoplasmic proteins.