Figure 4.
MDM2DD inhibited apoptosis and decreased p53 expression in MMTV/neu tumor. (A–C) Histological patterns of MMTV/neu (A), MMTV/neu/MDM2DD (B) and MMTV/neu/MDM2 (C) tumors. (D–F) Immunohistochemical analysis of MDM2 expressions in MMTV/neu (D), MMTV/neu/MDM2DD (E) and MMTV/neu/MDM2 (F) tumors. (G, H) TUNEL analysis in MMTV/neu (G) and MMTV/neu/MDM2DD (H) tumors. Arrows indicated the apoptotic cells. (I, J) Immunohistochemical analysis of p53 expressions in MMTV/neu (I) and MMTV/neu/MDM2DD (J) tumors. (K) Apoptotic indices of MMTV/neu and MMTV/neu/MDM2DD mammary tumors. (L) Immunoblot analysis of p53, p21 and FOXO3a expressions in mammary tumors from MMTV/neu, MMTV/neu/MDM2DD and MMTV/neu/MDM2 mice.