Figure 2. Panobinostat abrogates bortezomib-induced aggresome formation in JeKo-1 and Granta-519 cells.

A, JeKo-1 cells were treated with BZ, PS and a combination of the two for 16 hours and stained for HDAC6 and ubiquitin. Following this, immunofluroescent microscopy was performed using LSM-510 confocal microscope using a 63X/1.2 W correction objective. B, JeKo-1 and Granta-519 cells were treated with BZ, PS or a combination of the two drugs and the percentage of cells with aggresomes were counted. Values were plotted as the percentage of aggresome positive cells ± standard deviation. The p value for BZ versus the co-treatment is less than or equal to 0.005. C, JeKo-1 cells were treated with indicated doses of PS and BZ and the ultrastructure of cells was imaged using a transmission electron microscope. Aggresomes and ER dilatation are indicated by arrows and depicted as insets.