Figure 3. Treatment with Panobinostat induces ER stress, results in dose-dependent apoptosis and abrogates hsp90 client proteins in MCL cells.

A, JeKo-1 and Z18C cells were treated with indicated doses of PS for 6 hours and the mRNA expression of XBP1s, XBP1u, Erdj4, CHOP and NOXA was assessed by RT-PCR. The expression of β-actin served as a loading control. B, JeKo-1 and Z138C cells were treated with indicated doses of PS for 6 hours and the expression of ATF4, GRP78 and CHOP was assessed by immunoblotting. C, JeKo-1, MO2058, Z138C and Granta-519 cells were exposed to the indicated doses of PS for 48 hours and apoptosis was monitored by Annexin V and PI staining followed by flow cytometry. D, JeKo-1 cells were exposed to indicated doses of PS for 6 hours and the expression of c-RAF, AKT, Cyclin D1, CDK, survivin, c-Myc, BAK, BAX and β-actin were monitored by immunoblot analyses.