Gal3 protein is upregulated in human ALS spinal cord.
(A) Immunoblotting analysis of homogenized spinal cords from 9 ALS patients, 2 controls (normal individuals) and 15 other neurological diseases (ND), stained with antibodies against GAPDH and Gal3 (rabbit polyclonal Ab).
(B) Quantitative analysis of Gal3 level in all cases, normalized by the intensity of GAPDH.
(C) Validation of the immunoblotting results using another Gal3 antibody (mouse monoclonal Ab).