Fig. 2.

HPLC tracing of three serial blood samples, a standard (visible only on the UV channel, not shown) and urine sample from patient 1. The main urine peak (3,600 s) is actually well off scale (max. 310). Urine metabolites are observed in the range 3,200–3,400 s. The main peaks (650, 1,400, 2,150 and 3,600 s) coincide precisely with the retention of authentic EF5, determined by spiking individual samples with authentic EF5 (data not shown)