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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: J Orthop Res. 2010 Nov;28(11):1425–1430. doi: 10.1002/jor.21164

Figure 2.

Figure 2

Mutant SH3BP2 increases PLCγ2 phosphorylation by immunoblot. Levels of PLCγ2 Tyr1217 phosphorylation and total PLCγ2 and levels of PLCγ1 Tyr783 phosphorylation and total PLCγ1 were examined in response to transient transfection of mutant or wild-type SH3BP2 in RAW 264.7 cells. β-actin is used as a control to ensure that equal protein was loaded in each lane. This experiment was repeated three times with the same results. WT represents cells transiently transfected with wild-type. VO represents cells transiently transfected with vector only plasmid with no SH3BP2 insert.