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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: J Orthop Res. 2010 Nov;28(11):1425–1430. doi: 10.1002/jor.21164

Figure 3.

Figure 3

Mutant SH3BP2 does not increase Syk or Vav3 phosphorylation relative to the wild-type. Levels of Syk phosphorylation and total Syk and levels of Vav3 phosphorylation were examined in response to transient transfection of mutant or wild-type SH3BP2 in RAW 264.7 cells. β-actin is used as a control to ensure that equal protein was loaded in each lane. The mutant SH3BP2s are not more effective at Syk Tyr525/526 or Vav3 phosphorylation compared to the wild-type. This experiment was repeated three times with the same results. WT represents cells transiently transfected with wild-type SH3BP2. VO represents cells transiently transfected with vector only plasmid with no SH3BP2 insert.