Skip to main content
. Author manuscript; available in PMC: 2010 Oct 4.
Published in final edited form as: J Biol Chem. 2006 Oct 24;281(51):39507–39516. doi: 10.1074/jbc.M608551200

FIGURE 5. Heparin affinity chromatography comparing variable regions of collagen α1(XI).

FIGURE 5

Upper panel, elution profiles are shown for Npp[p6a78] (dotted and dashed line), Npp[p6b7] (dashed line), and Npp[p7] (solid bold line). Absorbance at 280 nm was normalized to the maximum height of the Npp[p6b7] peak. Conductivity during NaCl gradient elution of the protein is indicated on the right-hand axis (solid line). Lower panel, SDS-polyacrylamide gels of alternating 1-ml fractions collected during elution of the collagen α1(XI) splice variants. The position of the splice variants Npp[p6b7], Npp[p7], and Npp[p6a78] are indicated. The position of the bands in the lower panel are shown in relation to the chromatograms in the upper panel.