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Far-UV CD spectra of Npp[p7] and Npp[p6b7] are shown. CD spectra showing overlay of Npp[p7], 4.78 mg/ml, with (gray dashed line) and without (gray solid line) 0.25 mg/ml heparan sulfate and overlay of Npp[p6b7], 3.40 mg/ml, with (black dashed line) and without (black solid line) 0.25 mg/ml heparan sulfate. Note subtle change in spectra in the presence of heparan sulfate indicting a minor change in periodic secondary structure of the protein upon binding.
