TABLE 1. Mutagenic primer pairs.

Site-directed alteration of amino acid residues within putative binding site 1 147-KKKITK-152 is shown. Mutagenic primers are shown for each targeted change.

Wild type	pET11aVO	5′-GACAATGATTGTCGATTGTAAGAAGAAAATCACAAAGCCCCTCGATAGAAG-3′
Mutagenic primer pair K147A	CMB226S	5′-GACAATGATTGTCGATTGTAAGAAGAAAATCACAAAGCCCCTCGATAGAAG-3′
	CMB227A	5′-CTTCTATCGAGGGGCTTTGTGATTTTCTTCGCACAATCGACAATCATTGTC-3′
Mutagenic primer pair K148A	CMB228S	5′-GACAATGATTGTCGATTGTAAGGCGAAAATCACAAAGCCCCTCGATAGAAG-3′
	CMB229A	5′-CTTCTATCGAGGGGCTTTGTGATTTTCGCCTTACAATCGACAATCATTGTC-3′
Mutagenic primer pair K149A	CMB230S	5′-GACAATGATTGTCGATTGTAAGAAGGCAATCACAAAGCCCCTCGATAGAAG-3′
	CMB231A	5′-CTTCTATCGAGGGGCTTTGTGATTGCCTTCTTACAATCGACAATCATTGTC-3′
Mutagenic primer pair K152A	CMB232S	5′-GACAATGATTGTCGATTGTAAGAAGAAAATCACAGCGCCCCTCGATAGAAG-3′
	CMB233A	5′-CTTCTATCGAGGGGCGCTGTGATTTTCTTCTTACAATCGACAATCATTGTC-3′