Wild type |
pET11aVO |
5′-GACAATGATTGTCGATTGTAAGAAGAAAATCACAAAGCCCCTCGATAGAAG-3′ |
Mutagenic primer pair K147A |
CMB226S |
5′-GACAATGATTGTCGATTGTAAGAAGAAAATCACAAAGCCCCTCGATAGAAG-3′ |
|
CMB227A |
5′-CTTCTATCGAGGGGCTTTGTGATTTTCTTCGCACAATCGACAATCATTGTC-3′ |
Mutagenic primer pair K148A |
CMB228S |
5′-GACAATGATTGTCGATTGTAAGGCGAAAATCACAAAGCCCCTCGATAGAAG-3′ |
|
CMB229A |
5′-CTTCTATCGAGGGGCTTTGTGATTTTCGCCTTACAATCGACAATCATTGTC-3′ |
Mutagenic primer pair K149A |
CMB230S |
5′-GACAATGATTGTCGATTGTAAGAAGGCAATCACAAAGCCCCTCGATAGAAG-3′ |
|
CMB231A |
5′-CTTCTATCGAGGGGCTTTGTGATTGCCTTCTTACAATCGACAATCATTGTC-3′ |
Mutagenic primer pair K152A |
CMB232S |
5′-GACAATGATTGTCGATTGTAAGAAGAAAATCACAGCGCCCCTCGATAGAAG-3′ |
|
CMB233A |
5′-CTTCTATCGAGGGGCGCTGTGATTTTCTTCTTACAATCGACAATCATTGTC-3′ |