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Enzyme Kinetics of Variant KARS Proteins in Aminoacylation Assays
| KARS Enzyme | Km (μM) | kcat (s−1) | kcat/Km (s−1μM−1) | Relative Activity |
|---|---|---|---|---|
| Wild-type | 1.8 ± 0.9 | 1.4 ± 0.1 | 0.8 | 1 |
| p.Leu133His | 5.8 ± 2.4 | 0.3 ± 0.3 | 0.05 | 0.06 |
| p.Ile302Met | 2.1 ± 0.8 | 2.7 ± 1.6 | 1.3 | 1.6 |
| p.Thr623Ser | 3.5 ± 0.1 | 4.0 ± 0.7 | 1.1 | 1.4 |
