Figure 6.

Wdr11 Expression during Murine Development

(A–E) DIG-labeled whole-mount in situ hybridization with a Wdr11 antisense probe at different embryonic stages. High expression levels are found in all structures of the developing brain as early as E10.5. Expression in the limbs is prominent at E12.5 and E13.5. Staining was also observed in both the hind and forelimb buds, but as limbs developed, it shifted toward the terminal phalanges. At E14.5 the olfactory bulb and the developing cortex show the highest expression levels. A magnification of the developing cortex and olfactory bulb is shown in (E).

(F–H) Expression of Wdr11 in the adult brain. [35S]-UTP-labeled in situ hybridizations show prominent Wdr11 signals in the piriform cortex (F) as well as in the hippocampus and cerebellum (G). Note the higher signal intensity in the hypothalamic region within the dotted rectangle in (G). Single cells as well as clusters of neurons within the hypothalamic nuclei also showed Wdr11 expression in DIG-labeled cryosections (H). Signals were absent with the sense control. Abbreviations are as follows: tel, telencephalic vesicle; wh, wall of hindbrain; di, diencephalon; mes, mesencephalon; rho, rhombencephalon; sc, spinal cord; lb, limb bud; hl, hind limb; fl, fore limb; ob, olfactory bulb; pc, piriform cortex; gcl, granule cell layer of the cerebellum; hp, hippocampus; and hy, hypothalamus.