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Schematic illustration for the one-pot three-enzyme preparative-scale synthesis of Lewis x trisaccharide Galβ1-4(Fucα1-3)GlcNAcβProN3 from fucose, ATP, GTP and type II disaccharide Galβ1-4GlcNAcβProN3 (LacNAcβProN3). Enzymes used: FKP, a recombinant bifunctional l-fucokinase/GDP-fucose pyrophosphorylase from B. fragilis strain NCTC9343; HhFT1, H. hepaticus α1-3-fucosyltransferase 1; PpA, a recombinant inorganic pyrophosphatase from P. multocida.
