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The pH profile of MBP–HhFT1–His6 when LacNAcβPro2AA (filled diamonds) or Neu5Acα2-3LacNAcβPro2AA (dashed line with unfilled diamonds) was used as an acceptor substrate. Buffers used: MES (pH 5.0–6.5), HEPES (pH 7.0–8.0), Tris-HCl (pH 8.5–9.0), N-cyclohexyl-2-hydroxyl-3-aminopropanesulfonic acid (CAPSO) (pH 10), and N-cyclohexyl-3-aminopropanesulfonic acid (CAPS) (pH 10.0–11.0).
