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Kinetic parameters of recombinant MBP–HhFT1–His6
| Substrates | Km (mM) | kcat (min-1) | kcat/Km (mM-1 min-1) | |
|---|---|---|---|---|
| Fucosyltransferase | LacNAcβPro2AA | 45 ± 2 | 19 ± 1 | 0.43 |
| GDP-fucose | 2.4 ± 0.4 | 2.3 ± 0.1 | 1.0 | |
| Neu5Acα2-3LacNAcβPro2AA | 40 ± 1 | 25 ± 1 | 0.63 | |
| GDP-fucose | 3.3 ± 0.6 | 3.6 ± 0.2 | 1.1 | |
| GDP-Fucose hydrolysis | GDP-fucose | 13 ± 2 | 48 ± 2 | 3.7 |
