Fig. 5.

Functional complementation of Saccharomyces PMM temperature-sensitive PMM mutant (HMSF33 sec53-6 strain) by Gl-PGM2 but not by Gl-PGM1. The Saccharomyces sec53 gene encodes phosphomannomutase (PMM). (A) At 25°C (the permissive temperature), the mutant strain with no vector or transformed with Gl-PGM1, Gl-PGM2 or a functional PMM gene (Sc-Sec53) all grow normally. (B) At 37°C (nonpermissive or restrictive temperature), the mutant and the mutant transformed with Gl-PGM1 cannot grow. In contrast, the mutant transformed with Gl-PGM2 or with the functional PMM (Sc-Sec53) both grow at 37°C. These results show that the Gl-PGM2 has PMM activity but the Gl-PGM1 does not