TABLE 1.
Testis cII Mutation Frequencies in AA- or GA-Treated Male Big Blue Mice
| Group | Total plaques screened (×103) | Mutant plaquesa | MFb (×10−6) | Independent mutations | Mutation frequencyc (×10−6) |
| Control | 1206 | 7 | 5.8 | 7 | 5.8 |
| 1133 | 3 | 2.6 | 3 | 2.6 | |
| 1245 | 4 | 3.2 | 4 | 3.2 | |
| 1387 | 6 | 4.3 | 5 | 3.6 | |
| 1168 | 2 | 1.7 | 2 | 1.7 | |
| 1357 | 8 | 5.9 | 4 | 2.9 | |
| 1577 | 5 | 3.2 | 5 | 3.2 | |
| Mean ± SD | 3.8 ± 1.6 | 3.3 ± 1.3 | |||
| AA—1.4mM | 659 | 10 | 15.2 | 9 | 13.7 |
| 672 | 46 | 68.5 | 9 | 13.4 | |
| 753 | 8 | 10.6 | 6 | 8.0 | |
| 1042 | 29 | 27.8 | 4 | 3.8 | |
| 571 | 6 | 10.5 | 4 | 7.0 | |
| 786 | 6 | 7.6 | 6 | 7.6 | |
| Mean ± SD | 23.4 ± 25.6 | 8.9 ± 3.9d | |||
| AA—7.0mM | 795 | 15 | 18.9 | 13 | 16.4 |
| 660 | 12 | 18.2 | 7 | 10.6 | |
| 1086 | 13 | 12.0 | 10 | 9.2 | |
| 577 | 11 | 19.1 | 7 | 12.1 | |
| 989 | 14 | 14.2 | 10 | 10.1 | |
| 766 | 11 | 14.4 | 9 | 11.7 | |
| Mean ± SD | 16.1 ± 3.0 | 11.7 ± 2.5d | |||
| GA—1.4mM | 892 | 8 | 9.0 | 6 | 6.7 |
| 683 | 14 | 20.5 | 9 | 13.2 | |
| 660 | 8 | 12.1 | 6 | 9.1 | |
| 735 | 8 | 10.9 | 7 | 9.5 | |
| 908 | 11 | 12.1 | 9 | 9.9 | |
| 1376 | 9 | 6.5 | 9 | 6.5 | |
| Mean ± SD | 11.9 ± 4.7 | 9.2 ± 2.4d | |||
| GA—7.0mM | 405 | 9 | 22.2 | 8 | 19.8 |
| 428 | 20 | 46.7 | 16 | 37.4 | |
| 688 | 12 | 17.4 | 11 | 16.0 | |
| 635 | 15 | 23.6 | 10 | 15.7 | |
| 1238 | 13 | 10.5 | 13 | 10.5 | |
| 1009 | 11 | 10.9 | 11 | 10.9 | |
| Mean ± SD | 21.9 ± 13.3 | 18.4 ± 9.9d |
a
Sibling mutants or clonal expansions isolated from a single mouse with same type of mutations were included.
b
The MF is defined as the total number of mutant plaques divided by the total number of plaques screened.
c
The mutation frequency is defined as the number of independent mutations divided by the total number of plaques screened.
d
Significantly different from control at p < 0.01 (ANOVA, followed by the Holm-Sidak test).