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. Author manuscript; available in PMC: 2011 Oct 10.
Published in final edited form as: Eur J Pharmacol. 2010 Jun 30;644(1-3):10–16. doi: 10.1016/j.ejphar.2010.06.023

Fig. 5. The effect of submaximum AR-C69931MX on ADP-induced concentration-response curves of wild-type P2Y12 receptors (panel A) and mutants with mutated residues in TM3 (F104S) (panel B) and TM7 (S288P) (panel C) in antagonist analysis.

Fig. 5

Fig. 5

Inhibition of cAMP was measured after incubation of cells with different concentrations of ADP (0.1-30 μM) and stimulated for 5 min with or without 30 nM AR-C69931MX in presence of 20 μM forskolin. Data were normalized to the maximal response obtained in the absence of agonist and antagonist, which served as control (mean ± S.E.M., n=3). ## P<0.01, ### P<0.001, significant differences vs. respective control without agonist and antagonist. ** P<0.01, *** P<0.001, significant differences vs. respective values in the absence of the antagonist. (ANOVA followed by the Bonferroni post-test).