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. 2010 Sep 26;2010:872180. doi: 10.4061/2010/872180

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Copyright © 2010 Irina S.-R. Waisertreiger et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Intracellular localization and induction of ITPA. (a) The effect of HAP on ITPA levels and distribution in the WI-38, HCT116 and P32T cells. Immunofluorescence of ITPA in the WI-38, upper row, HCT116, middle row and P32T, lower row. Immunostaining and counterstaining were performed with antirabbit monoclonal antibody (green) and DAPI (blue), respectively. Bar—10 μm Representative images are shown. (b) Details of immunofluorescence pattern of ITPA in the HAPtreated WI-38 (upper row, 1.32 mM HAP) and P32T (lower row, 0.66 mM HAP) cells. Left column—DAPI only, middle column—detection of ITPA antibodies, and right column—merged picture.