Figure 1. PKC activity required for HSV-1 replication.
Replicate cultures of HEp-2 cells were infected at an MOI of 5 with HSV-1(F) A) Cells were treated with DMSO or 10uM pan-PKC inhibitor bis-indolylmaleimide (BIM I) beginning at five hours post infection (hpi). Titer was determined at 24 hpi. Virus yields are expressed as plaque forming units (PFU) per milliliter. Each data point represents the mean of five experiments. Error bars indicate standard deviations. p-values were determined via a paired Student T-Test.