Figure 4.

Four insertion lines in AtTRS120, a putative TRAPPII tethering factor. The top line represents the AtTRS120 gene. A single open reading frame is depicted by a line, and the exons are drawn as boxes. Four alleles, depicted with triangles, show a cytokinesis-defective phenotype (Table III). The scale is for nucleotides in kb. A line above the gene designates the interval spanned by RT-PCR primers, with which no transcripts were detected downstream of the four insertions. The bottom line represents the AtTRS120 protein. The Trs120p signature (designated by a gray arrow) spans the entire length of the protein, with five well-conserved domains (designated by black rectangles) characterized by the presence of highly conserved amino acids in the alignment (Cox et al., 2007). The scale is for amino acid (a.a.) residues. The black arrow represents a functional break point in yeast, with mutations upstream of the break point being lethal and mutations downstream being temperature sensitive (Cox et al., 2007). Note that all four insertions are upstream of the functional break point.