Table I. TRAPP and GARP subunits and their Arabidopsis homologs.
The amino acid conservation between the Arabidopsis proteins and their yeast and mammalian homologs is given in the last four columns. Alignment data were obtained from pairwise, global alignments of the protein sequences by EMBOSS (Rice et al., 2000 [Blossom62; gap open, 10.0; gap extend, 0.5]). Our phylogenetic analyses are consistent with the published data of Koumandou et al. (2007) and of Cox et al. (2007). % Sim., Percent similar; % Ident., percent identical. Dashes indicate that the yeast gene has no homolog in mammals or in Arabidopsis, or that the column header does not apply.
| Yeast Subunit | Mammalian Subunit | TRAPP Complex | Arabidopsis Homolog | GenBank Accession No. |
Saccharomyces cerevisiae |
Homo sapiens |
||
| % Sim. | % Ident. | % Sim. | % Ident. | |||||
| Bet5 | C1 | I and II | At1g51160 | 841539 | 39.5 | 23.1 | 53.9 | 37.1 |
| Trs20 | C2 | I and II | At2g20930 | 816627 | 49.2 | 31.6 | 69.3 | 49.3 |
| Bet3 | C3 | I and II | At5g54750 | 835565 | 67.0 | 42.8 | 70.4 | 50.0 |
| Trs23 | C4 | I and II | At5g02280 | 831680 | 38.5 | 24.3 | 46.8 | 33.2 |
| Trs31 | C5 | I and II | At5g58030 | 835915 | 37.0 | 26.3 | 65.0 | 45.7 |
| Trs33 | C6 | I and II | At3g05000 | 819661 | 32.2 | 20.3 | 58.2 | 42.4 |
| Trs85 | KIAA1012 | I and II | At5g16280 | 831488 | 17.8 | 9.3 | 40.2 | 23.7 |
| Trs65 | – | II | – | – | – | – | – | – |
| Trs120 | C9 | II | At5g11040 | 830971 | 33.2 | 18.6 | 36.2 | 20.0 |
| Trs130 | C10 | II | At5g54440 | 835532 | 34.6 | 17.3 | 31.6 | 19.1 |
| Vps52 | Vps52/SACM2L | – | At1g71270 | 843471 | 38.0 | 19.9 | 56.3 | 33.6 |
| Vps53 | Vps53 | – | At1g50500 | 841472 | 37.8 | 18.8 | 51.3 | 30.9 |
| Vps54 | Vps54 | – | At4g19490 | 827690 | 31.4 | 17.2 | 41.5 | 23.8 |