Table II. Insertion lines used in this study.
n = refers to the total number of individuals analyzed. Asterisks indicate that homozygous mutant individuals were recovered.
| Locus | Polymorphism | Position in Gene | Accession No. | Allele | Cytokinesis Defectivea | Transmissionb |
| TRAPPI | ||||||
| Bet3 | GABI_318C08 | Fourth intron | N430464 | bet3-1 | 0% (n = 200) | 7.6% (n = 66) |
| Bet5 | SALK_099482 | Second intron | N599482 | bet5-1 | 1% (n = 192) | 15.2% (n = 79) |
| SAIL_634_G07 | Second intron | N827313 | bet5-2 | 1% (n = 98) | 67.2%* (n = 64) | |
| Trs31 | FLAG_488E06 | Third intron | EWSTV26T3 | trs31-1 | 1% (n = 211) | 13.6% (n = 59) |
| Trs33 | SALK_109244 | Third exon | N609244 | trs33-1 | 5.7% (n = 614) | 55.6% (n = 36) |
| SALK_109724 | Third intron | N609724 | trs33-2 | 2.9% (n = 104) | 15.9% (n = 69) | |
| TRAPPII | ||||||
| Trs120 | SALK_125246 | First intron | N625246 | trs120-1 | 6.3% (n = 221) | 11.8% (n = 34)c |
| SALK_021904 | First intron | N521904 | trs120-2 | 8.4% (n = 455) | 7.7% (n = 78) | |
| SALK_111574 | Seventh exon | N611574 | trs120-3 | 11% (n = 349) | 13.1% (n = 107) | |
| SAIL_1285_D07 | Seventh intron | N879232 | trs120-4 | 11% (n = 617) | 23.8% (n = 105) | |
| GARP | ||||||
| Vps52 | SALK_055433 | 16th exon | N555433 | vps52-2d | 0% (n = 169) | 50% (n = 56) |
| Vps53 | SAIL_87_D06 | Fifth intron | N870946 | vps53-2 | 0% (n = 540) | 27.5% (n = 69) |
| GABI_400C01 | Seventh exon | N344145 | vps53-3 | 0% (n = 620) | 82% (n = 62) | |
| SAIL_117_D11 | 13th intron | N871277 | vps53-4 | 0% (n = 221) | 50.8% (n = 59) | |
| GABI_463D10 | 23rd exon | N344181 | vps53-5 | 0% (n = 600) | 87.8%* (n = 49) | |
| Vps54 | SALK_036485 | First exon | N536485 | vps54-1d | 0% (n = 196) | 25.5% (n = 55) |
| SALK_062261 | Eighth exon | N562261 | vps54-2d | 0% (n = 340) | 39.1% (n = 69) | |
Cytokinesis-defective mutant seedlings on the plate in the progeny of a selfed hemizygous line (%). In the absence of defects in transmission of the mutant alleles, 25% would be expected.
Hemizygous or homozygous segregants in the progeny of a hemizygous line grown on soil (%). In the absence of defects in transmission of the T-DNA insertion, and in case of lethality, 67% would be expected. All numbers are based on PCR analysis of genomic DNA isolated from soil-grown individuals (Supplemental Fig. S1), except as noted in subsequent footnotes.
For trs120-1, we scored cytokinesis-defective mutants in the progeny of individual lines.
These alleles have been described by Guermonprez et al. (2008).