Figure 4.

Structures of the starch metabolism genes and positions of the nonsilent mutations discovered in the forward genetic screen and by TILLING. Boxes and lines indicate exons and introns, respectively. Only the TILLING mutations leading to a starch phenotype and/or predicted by the PARSESNP program to have position-specific scoring matrix values greater than 0 are shown. The length of the gene sequence (in bp) is given on the right. The symbols above the gene specify the mutation type and position: octagons, stop codons; diamonds, splice site junction mutations; triangles, amino acid changes. Details of the nature and position of the mutations are given in Supplemental Table S6.