Table I. Starch content at the end of the day in leaves of mutants with starchless or low-starch phenotypes.
Genotypea | Starch Content | |
Wild Type | Mutant | |
mg Glc equivalents g1 fresh wt | ||
PGM1 | ||
pgm1-3 | 25.3 ± 6.4 | n.d. |
pgm1-4 | 20.1 ± 4.8 | n.d. |
pgm1-5 | 29.7 ± 6.2 | 6.1 ± 1.3 |
PGI1 | ||
pgi1-1 | 17.2 ± 5.9 | 1.83 ± 0.35 |
pgi1-2 | 50.6 ± 4.7 | 4.56 ± 1.90 |
APS1 | ||
aps1-1 | 47.0 ± 4.1 | 3.73 ± 1.29 |
APL1 | ||
apl1-1 | 59.6 ± 10.5 | 12.7 ± 2.0 |
For each genotype, F3 homozygous mutant and wild-type plants for analysis were selected by genotyping individual plants in a single F2 population derived from a cross between the original mutant in Gifu and MG-20. Plants were grown for 7 weeks, as described for starch quantification in “Materials and Methods.”