Cold sensitivity of chs2 mutant plants. A, Phenotypes of wild-type Col and chs2 plants grown in soil at 22°C for 4 weeks (top row), cold treated at 4°C for 10 d (middle row), followed by 22°C for 3 d (bottom row). B, Phenotypes of wild-type Col and chs2 plants grown in soil at 22°C for 6 weeks (top) followed by cold treatment at 4°C for 10 d (bottom). C and D, Phenotypes of Col and chs2 plants directly geminated on MS plates and grown at 4°C for 3 months (C) or grown at 22°C for 3 weeks and then transferred to 4°C for an additional 10 d (D). E, Phenotypes of wild-type Col and chs2 plants grown in soil at 16°C for 7 weeks. F, Phenotypes of chs2 plants grown on MS plates at the indicated temperatures for 4 weeks. Images are of representative plants.