Figure 7.

Phenotypes of the chs2 double mutants under cold conditions. Three-week-old 22°C-grown plants were treated at 4°C for 6 d (C–E), 14 d (A), or 5 weeks (B). A and B, Growth phenotypes of the double mutants under cold conditions. Representative plants are shown. C, Trypan blue staining of the leaves from the double mutants. Bar = 100 μm. Note that the photographs of 4°C-treated Col and chs2 plants stained with trypan blue are identical to those shown in Figure 2B. D, DAB staining of the leaves from the double mutants. Bar = 100 μm. E, PR1 gene expression in the double mutants by real-time PCR. The data represent means of three replicates ± sd. * P < 0.01 (t test), significant difference from chs2. All experiments were repeated three times with similar results.